Journal: Oncotarget

Article Title: MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7.

doi: 10.18632/oncotarget.5224

Figure Lengend Snippet: Figure 2: The expressions of CASP3 and CASP7 mRNA are down regulated in NSCLC. a. CASP7 mRNA expressions in thirty paired NSCLC tissues and NATs. b. CASP3 mRNA expressions in thirty paired NSCLC tissues and NATs. GAPAH was used for normalization. c. and d. Kaplan-Meier plots of overall survival of lung cancer patients, stratified by expression of CASP7 (1926 patients) c. or CASP3 (1926 patients) d.. Data obtained from the Kaplan-Meier plotter database (kmplot.com/analysis).

Article Snippet: nsclc tissue samples Human Lung Tissue Microarrays (IMH-358) were purchased from Novus Biologicals, San Diego, CA.

Techniques: Expressing

Journal: Oncotarget

Article Title: MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7.

doi: 10.18632/oncotarget.5224

Figure Lengend Snippet: Figure 4: Expressions of CASP3 and CASP7 are inversely correlated with miR-224. a. Representative pictures and summary of co-expression analyses for miR-224 and CASP7 in NSCLC tissues. MiR-224 was detected by using 5’-DIG labeled LNA probe (purple) and CASP7 was detected by immunohistochemistry (red). Left: High CASP7 and low/neg miR-224, Middle: similar expression of CASP7 and miR-224, right: Low/neg CASP7 and high miR-224. b. Representative pictures and summary of co-expression analyses for miR- 224 and CASP3 in NSCLC tissues. MiR-224 was detected by using 5’-DIG labeled LNA probe (purple) and CASP3 was detected by immunohistochemistry (red). Left: High CASP3 and low/neg miR-224, Middle: similar expression of CASP3 and miR-224, right: Low/ neg CASP3 and high miR-224. c. In the cases of NSCLC where both miR-224 and CASP7 expression were noted, no detectable CASP7 was found in cancer cells overexpressing miR-224 (purple arrow).

Article Snippet: nsclc tissue samples Human Lung Tissue Microarrays (IMH-358) were purchased from Novus Biologicals, San Diego, CA.

Techniques: Expressing, Labeling, Immunohistochemistry

Journal: Translational Oncology

Article Title: Vasorin/ATIA Promotes Cigarette Smoke–Induced Transformation of Human Bronchial Epithelial Cells by Suppressing Autophagy-Mediated Apoptosis

doi: 10.1016/j.tranon.2019.09.001

Figure Lengend Snippet: Increased vasorin expression in human lung cancer and HBECs exposed to tobacco carcinogens . (A) Tissue arrays were stained for vasorin with immunohistochemistry. The normal airway and alveolar epithelial cells are weakly positive while the tumor cells are strongly positive. (B) Results of tissue arrays with comparison of the staining between vasorin and normal lung epithelial cells. (C) Vasorin was examined in nontransformed (HBEC-1 and -2) and lung cancer cell lines. Relative vasorin fold increase (HBEC-2 was set to 1) was shown. (D) BEAS-2B and HBEC-2 cells were treated with CSE for 24 hours. (E) HBEC-2B (BPDE-transformed) cells were compared with HBEC-2 cells for vasorin expression. Protein was detected by western blot in C to E with β-actin as loading control.

Article Snippet: Immunohistochemistry staining using VECTASTAIN ABC Kit and DAB (3,3′-diaminobenzidine) Peroxidase Substrate Kit (Vector Laboratories, Burlingame, CA) and result assessment of human lung cancer tissue array (Imgenex; Novus Biologicals, Centennial, CO) has been described previously [ ].

Techniques: Expressing, Staining, Immunohistochemistry, Comparison, Transformation Assay, Western Blot, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: YEATS2 promotes malignant phenotypes of esophageal squamous cell carcinoma via H3K27ac activated-IL6ST

doi: 10.3389/fcell.2025.1497290

Figure Lengend Snippet: YEATS2 stable knockdown inhibited the abilities of ESCC cells to proliferate and migrate in vitro and in vivo . (A) Western blot and RT-qPCR were performed to verify the efficiency of YEATS2 knockdown and overexpression acted by CRISPR/Cas9 and CRISPR/dCas9-SAM system. Data shown are the mean ± SD of three biological replicates. p value was calculated by unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (B) MTT was performed to detect YEATS2 effect on the proliferation ability of ESCC cells in vitro , including KYSE150 and TE9. Data shown are the mean ± SD of three biological replicates. p values were calculated by one-way ANOVA or unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (C–D) Colony Formation Assay was performed to detect YEATS2 effect on the colony forming ability of ESCC cells in vitro , including KYSE150 and TE9. Data shown are the mean ± SD of three biological replicates. p values were calculated by unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (E–F) Transwell was used to detect YEATS2 effect on the migration ability of ESCC cells in vitro , including KYSE150 and TE9. Data shown are the mean ± SD of three biological replicates. p values were calculated by unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (G–H) Scratch Wound Healing was used to detect YEATS2 effect on the wound healing ability of ESCC cells in vitro , including KYSE150 and TE9. Data shown are the mean ± SD of three biological replicates. p values were calculated by unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (I) Photographic images and hematoxylin–eosin (HE) staining of tumors from nude mice (n = 3 in each group). (J) The volume change of tumors from tumor-bearing nude mice. Tumor volume was calculated and diameters were measured at a regular interval of 6 days for up to 33 days. Data shown are the mean ± SD of three biological replicates. p values were calculated by unpaired t tests with * p < 0.05, ** p < 0.01, and *** p < 0.001. (K) Representative images of lungs from tail-vein lung metastatic models (n = 4 in each group) and HE staining for lungs from tail-vein lung metastatic models.

Article Snippet: We have constructed a subcutaneous tumorigenesis model and lung metastatic tumor model in 6-week-old BALB/c nude female mice (Gempharmatech Co.).

Techniques: Knockdown, In Vitro, In Vivo, Western Blot, Quantitative RT-PCR, Over Expression, CRISPR, Colony Assay, Migration, Staining